12/8/2023 0 Comments Vimr lab phageIndustry Insights with Yuning Chen on Recombinant Proteins.ExpertAnswers: Yuning Chen on Antibody Production.Universal Vaccine Advancement through AI and Recombinant Technology. Phage display is a selection technique based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection and phage propagation. It allows in vitro selection and production of recombinant monoclonal antibodies of high specificity and affinity. In 1985, Smith was first to discover that foreign DNA fragments can be fused to the gene encoded for pIII coat protein of a nonlytic filamentous phage and expressed as a fusion protein on the virion surface without disturbing the infectivity of the phage. Winter flipped the process of phage display by using the phage to display antibodies (rather than proteins) then fishing out the desired antibodies that bind to molecules or even cells. The invention of antibody phage display has revolutionised monoclonal antibody drug discovery.įor monoclonal antibody production, both hybridoma and phage display are very important technologies.
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